Katedra Genetyki i Ogˇlnej Hodowli Zwierz╣t
Department of Genetics and Animal Breeding
http://gen.ar.wroc.pl
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  2000

  •  Evidence for Heterogeneity in Recombination in the Human Pseudoautosomal Region: High Resolution Analysis by Sperm Typing and Radiation-Hybrid Mapping 
  • Sex ratio distortion in bovine sperm correlates to recombination in the pseudoautosomal region
  • Comparison of growth hormone and kappa-casein gene polymorphism in Polish Red and German Red cattle breed 
  • A note on transferrin polymorphism in the blood serum of arctic (Alopex lagopus) and silver (Vulpes vulpes) fox 
  •  Inheritance of blue coat colour intensity and genetic parameters of skin size and quality in arctic fox (Alopex lagopus L.)
  • Genetic, phenotypic and environmental trends of conformation traits in arctic fox Alopex lagopus (L.) 
  •  Additive genetic and error variance components for conformation and coat traits in arctic fox Alopex lagopus (L.)
  •  Genetic determination of reproduction traits in silver fox  (Vulpes vulpes
  •  Genetic parameters of coat colour in golden fox  (Vulpes vulpes L.)
  •  Effect of melanin biosynthesis on the coat colour of animals 

  •  Logistic regression approach to modelling the variability of recombination rate
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    Evidence for Heterogeneity in Recombination in the Human Pseudoautosomal Region:
    High Resolution Analysis by Sperm Typing and Radiation-Hybrid Mapping 

    Sigbj°rn Lien,1,4 Joanna Szyda,2 Birgit Schechinger,3 Gudrun Rappold,3 and Norm Arnheim4 

    1 Department of Animal Science, Agricultural University of Norway, Aas, Norway; 
    2 Department of Genetics, Wroclaw Agricultural University, Wroclaw, Poland; 
    3 Institute of Human Genetics, Karl-Ruprecht University of Heidelberg, Heidelberg, Germany; and 
    4 Molecular Biology Program, University of Southern California, Los Angeles 

    Received April 28, 1999; accepted for publication September 28, 1999; electronically published January 27, 2000.

    Summary

    Accurate genetic and physical maps for the human pseudoautosomal region were constructed by use of sperm typing and high-resolution radiation-hybrid mapping.
    PCR analysis of 1,912 sperm was done with a manual, single-sperm isolation method. Data on four donors show highly significant linkage heterogeneity among
    individuals. The most significant difference was observed in a marker interval located in the middle of the Xp/Yp pseudoautosomal region, where one donor showed a particularly high recombination fraction. Longitudinal models were fitted to the data to test whether linkage heterogeneity among donors was significant for multiple
    intervals across the region. The results indicated that increased recombination in particular individuals and regions is compensated for by reduced recombination in
    neighboring intervals. To investigate correspondence between physical and genetic distances within the region, we constructed a high-resolution radiation-hybrid map
    containing 29 markers. The recombination fraction per unit of physical distance varies between regions ranging from 13- to 70-fold greater than the genome-average
    rate.
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    Sex ratio distortion in bovine sperm correlates to recombination in the pseudoautosomal region








    JOANNA SZYDA 1, HENNER SIMIANER 2 and SIGBJRN LIEN 3

    1 Department of Animal Genetics, Wroclaw Agricultural University, Kozuchowska 7,     51-631 Wroclaw, Poland
    2  Department of Animal Husbandry and Animal Breeding, Animal Genetics Group, University of Hohenheim (470/HG), 70593 Stuttgart, Germany
    3 Department of Animal Science, Agricultural University of Norway, 1432 ?s, Norway

    (Received October 23, 1998; Revised May 28, 1999)

    Abstract

    A total of 2122 single sperm from 35 bulls belonging to six different paternal half-sib groups were analysed with respect to two markers in the bovine
    pseudoautosomal region (PAR) and sex- specific loci on the X and Y chromosomes, respectively. A segregation ratio significantly different from 1[ratio]1 was
    observed in a test over all families, with a higher proportion of X-bearing gametes (53í5%). The analysis of recombination conducted separately for X- and
    Y-bearing sperm showed that X-bearing sperm cells possess highly significant individual and between-family variability in recombination rate, whereas Y-bearing
    sperm show linkage homogeneity. To test whether the two phenomena are related, different logistic regression models were fitted to the data. The results show that
    sex ratio significantly correlates with changes in recombination rate among X-bearing but not among Y-bearing sperm. Different hypotheses to explain these
    observations are discussed.

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    http://www.journals.cup.org/live/00166723/abs/S0016672399004085h.htm



     

    Journal of Applied Genetics 41(3), 2000, 181-185.

    Comparison of growth hormone and kappa-casein gene polymorphism in Polish Red and German Red cattle breed

    Jind°ich ╚═TEK , Andrzej FILISTOWICZ , Vßclav ěEHOUT , Výra NEUBAUEROV┴ 

    Abstract: The polymorphism of kappa-casein (alleles A, B and E) and growth hormone (alleles L and V) genes was studied using PCR/RFLP method with the aim to evaluate the degree of genetic diversity of Polish Red and German Red cattle, which are endangered with extinction. At the kappa-casein locus, a very low frequency of allele B in German Red cattle was found. In both the breeds, allele E was absent. There was a relatively high frequency of allele L in the growth hormone locus, especially for German Red cattle. The results were compared with frequencies and diversities of other red cattle breeds.


    Animal Science Papers and Reports 18(3), 2000, 217-222.

    A note on transferrin polymorphism in the blood serum of arctic (Alopex lagopus) and silver (Vulpes vulpes) fox

    Magdalena Zato˝, Jacek Ku╝miak, Leokadia Bicka, Andrzej Filistowicz

    Abstract. The frequency of transferrin genotypes and alleles in the blood serum was analysed in 106 arctic foxes and 93 silver foxes. The electrophoresis was performed in 7.5% polyacrylamide gel. Three alleles were found: TfF , TfS and TfD. Variants F and S occurred in the arctic (0.604 and 0.396, respectively), while F and D in silver fox (0.758 and 0.242, respectively).






    Zeszyty Naukowe AR Wroc│aw, Zootechnika XLV, 362, 1999, 227-242

     Inheritance of blue coat colour intensity and genetic parameters of skin size and quality in arctic fox (Alopex lagopus L.)

    Wierzbicki Heliodor

    Abstract. The aim of the study was the attempt of determination of principles of blue coat colour intensity inheritance in Arctic foxes. Moreover, genetic parameters (coefficients of heritability, phenotypic, genetic and environmental correlations) of skin characteristics affecting its price at the auction-house were estimated. The animals taken for the study consisted of 1553 foxes bred on the Žniaty Fox Farm in the years 1997-1998. Genetic parameters were estimated with the use of REML method. Analysis of coat colour intensity among parents and progeny was performed in order to explain the rules of inheritance of fur coloration. The results obtained suggest that coat colour intensity is determined by polygenes and is inherited like a quantitative character. The genetic parameters of the skin traits indicate that effective breeding programmes basing on population genetic variability and correlations between skin traits can be applied. 



     

    Journal of Applied Genetics 41(2), 2000, 113-122.

    Genetic, phenotypic and environmental trends of conformation traits in arctic fox Alopex lagopus (L.)

    Wierzbicki H., Filistowicz A., Przysiecki P. 

    Abstract. Data on 3782 arctic foxes, bred on a Žniaty fox farm (Poland) in 1985-1998 were used to estimate the genetic, phenotypic and environmental trends of conformation traits. Body size (BS), colour type (CT), colour purity (CP), coat density (CD), hair length (HL), general appearance (GA) and total score (TS) were analysed. The BLUP with a single-trait animal model was used to estimate the genetic trends. The estimates of genetic and phenotypic trends were positive for BS, CT, CP, HL, GA, TS and ranged from 0.0006 to 0.0995 point/year and from 0.0100 to 0.1302 point/year, respectively, but were negative for CD (-0.0374 point/year and ľ0.0262 point/year, respectively). The environmental trends ranged from ľ0.0024 point/year for CT to 0.0304 point/year for TS.



     

    Scientifur 24(3), 2000, 217-222.

    Additive genetic and error variance components for conformation and coat traits in arctic fox Alopex lagopus (L.)

    Wierzbicki Heliodor 

    Abstract. Records of 3782 arctic foxes were used for estimation of additive genetic and error variance components for conformation and coat traits. Body size (BS), colour type (CT), colour purity (CP), coat density (CD), hair length (HL), general appearance (GA) and total score (TS) were analysed. The variance components were estimated by the Restricted Maximum Likelihood (REML) method with an univariate animal model. Since data exhibited skewed distribution (skewness ranged from 0.0110 for CP to ľ3.8631 for TS) the probit transformation was applied. Both transformed and untransformed data were used for the estimation of variance components. Transformation of the data resulted in reduced estimators of variance components, and in consequence, lower heritability estimates for studied traits were obtained (heritabilities ranged from 0.266 for GA to 0.533 for HL). 



     

    Animal Science Papers ans Reports, 18(3), 2000, 209-216.

    Genetic determination of reproduction traits in silver fox 
    (Vulpes vulpes

    Przysiecki P., Wierzbicki H., Filistowicz A.

    Abstract. Reproductive performance of 1304 males (date of birth, first and last mating in the season, age of reproductive maturity, length of reproductive season) and 2914 females (date of birth, mating, littering, age of reproductive maturity, length of pregnancy, number of pups born and reared and pup losses) of silver fox during the first reproductive season was evaluated. Heritability of the features and genetic and phenotypic correlations between traits were estimated. The results suggest considering all analysed features in males and females selection directed towards high fertility, but birth term should be the first natural criterion in young animals selection. The other features should be essential criteria of culling animals due to poor reproduction.



     

    Journal of Applied Genetics 41(4), 2000,

    Genetic parameters of coat colour in golden fox 
    (Vulpes vulpes L.)

    Filistowicz A., Przysiecki P., Wierzbicki H.,
    Filistowicz A, Tokarska M.

    Abstract. Genetic parameters (heritability, genetic and phenotypic correlations) of chosen coat colour traits of golden fox were estimated. 1013 animals, born on the Žniaty fox farm (Poland) in 1985-1999 were evaluated. In 1993-1999 colour type was additionally assessed for 833 animals, by detailed evaluation of coat colour on the back and sides of the body, throat colour, belly colour and the amount of silver hair. The REML method was used to estimate genetic (co)variance components. Data were transformed using the probit transformation. Heritability estimates for coat traits were low (0.04 to 0.22). Values of most of the estimated genetic parameters (h2, rG, rP) were comparable to those frequently reported for other colour types of silver fox. 



     

    Medycyna weterynaryjna, 56(11),2000, 695-699.

    Effect of melanin biosynthesis on the coat colour of animals 

    Wierzbicki Heliodor

    Abstract. The formation of melanin pigment is a complex event requiring several enzymes and proteins, and the pigment-containing subcellular organelle, the melanosome. The process is regulated by many loci encoding proteins, that are required for the development of several cell lineages. The biosynthesis of melanin depends on the metabolism of amino acid L-tyrosine. The copper containing enzyme - tyrosinase is the major factor involved in the melanin chemical pathway which results in either black-brown eumelanin or red-yellow phaeomelanin. Generally, melanin biosynthesis is modulated by variety of hormones and neurotransmitters acting on transmembrane receptors located on the cell surface. Alpha-melanocyte-stimulating hormone (a -MSH), melanin-concentrating hormone (MCHA), melatonin and catecholamines are the most important pigment cell agonist. Growth factors such as basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF) and mast cell growth factor (MGF or KIT ligand) modulate the melanogenic processes as well. Mutations at loci encoding factors determining pigmentation, produce pleiotropic effects on viability as well as hematopoiesis and fertility.



    Szyda, J., Simianer, H. and Lien, S. 

    Logistic regression approach to modelling the variability of
    recombination rate.

    Journal of Animal Breeding and Genetics 117, 17-24.

    Abstract

    The objective of the paper is to quantify the relationship between
    recombination rate and factors such as: "sex of sperm", paternal halfsib
    family, and individual, using logistic regression modelling. The
    analysed data set consists of 2214 single bovine sperm cell samples.
    Haplotypes at each single sperm were determined at eleven marker loci
    forming eight intervals located on chromosomes 6, 23 and X/Y. The
    experimental design comprises six paternal halfsib families. Six
    logistic regression models are fitted to the data from each interval.
    Departure from commonly assumed homogenous recombination is detected for
    one marker interval on chromosome 23 - influence of individual and "sex
    of sperm" by family interaction, and for both intervals mapped to sex
    chromosomes - influence of "sex of sperm" and paternal halfsib family
     
     
    Montenegro.com NewsJoanna Szyda
    Montenegro.com NewsJoanna Szyda
    Montenegro.com NewsJind°ich ╚═TEK , Andrzej FILISTOWICZ , Vßclav ěEHOUT , VýraNEUBAUEROV┴
    Montenegro.com NewsMagdalena Zato˝, Jacek Ku╝miak, Leokadia Bicka, Andrzej Filistowicz
    Montenegro.com NewsWierzbicki Heliodor
    Montenegro.com NewsWierzbicki H., Filistowicz A., Przysiecki P. 
    Montenegro.com News.Wierzbicki Heliodor
    Montenegro.com NewsPrzysiecki P., Wierzbicki H., Filistowicz A.
    Montenegro.com NewsFilistowicz A., Przysiecki P., Wierzbicki H.,
    Filistowicz A, Tokarska M.
    Montenegro.com NewsWierzbicki Heliodor
    Montenegro.com NewsSzyda, J., Simianer, H. and Lien, S

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